Grow Microorganism With Brain Heart Infusion Broth

 Brain Heart Infusion Broth medium is a highly nutritive medium. It is used as a blood culture system for cultivating a wide variety of microorganisms. This culture medium complies with EN ISO 6888 and APHA standards. Brain Heart Infusion Broth Medium is a variation of Rosenow's original formulation, which included brain tissue pieces in dextrose broth. It is a non-selective, enriched broth medium that can be used to grow both fastidious and non-fastidious microorganisms. This medium will also allow aerobic microorganisms from clinical and non-clinical specimens to grow. Gentamicin and/or chloramphenicol are recommended for fungi-selective isolation.

Composition

Ingredients

Gms / Ltr

Calf Brain infusions from 200 gm

12.500

Peptone

10.000

Beef heart Infusion from 250 gms

5.000

Sodium chloride

5.000

Disodium hydrogen phosphate

2.500

Dextrose

2.000

Principle

Organic nitrogen, carbon, and vitamins are provided by the mixture of brain and heart infusions. The carbohydrate source is dextrose. To stimulate early growth, a low concentration of dextrose is used. The osmotic environment is maintained by sodium chloride. The buffering agent in this medium is sodium phosphate, which also helps neutralise the acids produced by dextrose utilisation and thus maintains viability.


TM Media is one of the leading manufacturers of culture media. TM Media offers Brain Heart Infusion Broth in a variety of forms. We offer Brain Heart Infusion Broth Base as a Dehydrated Culture Media with different concentrations of agar, NaCl, and PABA. The Brain Heart Infusion Broth with 6.5 % NaCl can be used for the selective cultivation of salt-tolerant microorganisms, and the Brain Heart Infusion with PABA and agar can be used for the culturing of blood from patients under sulphonamide therapy. We also offer a vegetarian range, Ready-to-Use tubes, and bottles of Brain Heart Infusion Broth.

How to Use Brain Heart Infusion Broth for Blood Culture?

  • Remove the plastic cap and disinfect the part of the rubber stopper that is now exposed.

  • Draw the patient’s blood with the sterile needle and syringe, and transfer the blood sample immediately into the culture bottle by puncturing the rubber stopper with the needle and injecting the blood.

  • Venting may be required for aerobic cultures but not for anaerobic cultures.

  • Incubate at 35–37 degrees Celsius for 18–48 hours, then for 7 days to confirm negative results.

The TM Media Brain Heart Infusion Broth is pre-sterilized; hence, sterilization is not required.

TM Media offers Dehydrated and Ready-To-Use Culture Media such as CLED, EMB, and BHI. Our laboratory culture media collection was designed for researchers, with a diverse selection of high-quality products to keep their lab running smoothly and effectively. Along with culture media, we also provide Laboratory Consumables, Plant Tissue Culture Media, Antibiotic Sensitivity Discs, and many more microbiology laboratory products.

For more, visit www.tmmedia.in.


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