Antibiotic Sensitivity Discs

 Antibiotics are widely available nowadays. Each antibiotic is only effective against one type or species of bacteria. Antimicrobial resistance is now a global issue, and this is creating the need for new antimicrobial compounds.

Antibiotic Sensitivity Test

Usually, in the pharmaceutical industry or a medical laboratory, an antibiotic sensitivity test is used to determine the susceptibility of the tested antibiotic to the test organism. It involves the use of culture methods that expose bacteria to antibiotics. In this method, the test organism is inoculated on the culture media, and antibiotic impregnated discs are placed on that bacterial lawn. This test is also called the disc diffusion test.

In this method, the test bacteria are inoculated on Muller-Hinton agar plates before the antibiotic-impregnated discs are placed on top. When an antibiotic-impregnated disc is placed on agar inoculated with the test bacterium, the antibiotic diffuses radially outward, creating an antibiotic concentration gradient. If the test bacterium is susceptible to the antibiotic, a clear zone or ring forms around the antibiotic disc after incubation.

The diameter of areas without bacterial growth, known as the "zone of inhibition," on agar culture plates that have been evenly inoculated with the test bacteria is frequently measured in this culture method. If the bacteria are susceptible, a clear ring or zone of inhibition appears around the antibiotic disc after incubation. Bacteria are classified as sensitive, intermediate, or resistant to an antibiotic by measuring and comparing the diameter of this zone of inhibition.  The size of the zone of inhibition can be used to calculate the minimum inhibitory concentration, which is the lowest antibiotic concentration that prevents bacterial growth.

Disc diffusion is the most basic and least expensive method for testing antibiotic susceptibility, and it can be easily adapted to test newly available antibiotics or formulations. The disc diffusion method is preferable for discovering alternative antibiotics. Multiple antibiotics can be tested simultaneously using the disc diffusion method.

This technique can be either qualitative or quantitative. A qualitative test indicates whether or not resistance exists, whereas a quantitative test uses a minimum inhibitory concentration (MIC) to describe the concentration of antibiotic to which a bacterium is sensitive.

TM Media Manufacturer Of Antibiotic Sensitivity Discs

TM Media is a market leader in the production of antibiotic discs. TM Media has over 70 antibiotic sensitivity discs as per CLSI concentration. They offer Antibiotic Sensitivity Discs like Ampicillin, Cefoxitin, Chloramphenicol, Levofloxacin, Linezolid discs, and many more in three different packaging formats: blister pack, cartridge, and vial, to meet your experimental needs.

Antibiotic combinations for gram-positive organisms, gram-negative organisms, Pseudomonas species, and UTI pathogenic microorganisms are available at TM Media.

Aside from Antibiotic Sensitivity Discs, TM Media offers Culture Media, Ready-to-Use Culture Media, Lab Consumables, and other products that aid the clinical and pharmaceutical industries in the fight against microbial infections.