Test The Sensitivity Of Microorganisms With Mueller Hinton Agar Plate

The Mueller-Hinton formulation was originally developed for the cultivation of pathogenic Neisseria species, but now it is widely used as an antimicrobial susceptibility test medium. It is recommended for the diffusion of antimicrobial agents impregnated on paper discs through an agar gel, as described in the CLSI-approved standard. The CLSI chose Mueller-Hinton Agar for a variety of reasons, including:

  1. It demonstrates good batch-to-batch reproducibility for susceptible testing.
  2. It is low in sulfonamide, trimethoprim, and tetracycline inhibitors.
  3. It promotes the growth of the majority of non-fastidious bacterial pathogens.
  4. Many data points and years of experience with its performance have been gathered.

Kirby-Bauer et al. suggested using this medium for antibiotic susceptibility testing with a single high-concentration disc. Because of its reproducibility, the WHO Committee on Standardization of Susceptibility Testing has approved Mueller-Hinton Agar for determining microorganism susceptibility. Mueller Hinton Agar with 5% sheep blood and Mueller Hinton Agar with hemoglobin have been recommended for antimicrobial susceptibility testing of Streptococcus pneumoniae and Haemophilus influenza.



The Kirby-Bauer method is based on the agar diffusion of antimicrobial substances imbedded on paper discs. This method employs a disc with a single concentration of antimicrobial agent, and the zone diameters observed are correlated with minimum inhibitory concentration (MIC) values. A standardized suspension of the organism is swabbed across the medium's entire surface. The medium's surface is then covered with paper discs impregnated with specific amounts of antimicrobial agents. The zones of inhibition around each disc are measured after incubation. The susceptibility of microorganisms to antibiotic agents is determined by comparing them with CLSI standards. The various factors that influence disc diffusion susceptibility tests are agar depth, disc potency, inoculum concentration, pH of the medium, and beta-lactamase production by the test organisms.

TM Media - Manufacturer of Ready-to-Use Mueller-Hinton Agar Plates

TM Media is a well-known Culture Media manufacturer. They offer a wide range of Culture Media and Biological Media Bases of both animal and non-animal origin. Culture media comes in two varieties: Dehydrated and Ready-to-Use.

TM Media’s Mueller-Hinton Agar Plates are a perfect choice for Antibiotic Sensitivity Testing by the Kirby-Bauer method. These 90-mm plates are easy-to-use and give accurate results.

Along with Culture Media, TM Media also offers Antibiotic Sensitivity Discs, Lab Consumables such as Nichrome Loop, Disposable Swabs, Petri Plates, and many more.

For more, visit www.tmmedia.in.


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