Yeast Extract Agar - The Highly Nutritive Medium


Introduction

Yeast extract agar is a highly nutritive medium containing yeast extract as a source of vitamins. It allows the cultivation of a wide spectrum of bacteria, yeasts, and molds. Yeast extract agar is formulated according to the formula described by Windle Taylor for the plate count of microorganisms in water. A large number of microorganisms, particularly those from soil and vegetation, can be found in water. This medium can also be used for teaching and demonstrations with simple organisms.

Make a decimal dilution bank with Ringer Solution from the water sample and transfer aliquots to two parallel series of plates. Pour in the cooled molten Yeast Extract Agar and homogenise with the sample. Incubate one of the plates for 24 hours at 35°C and the other plate for 3 days at 20–22°C. This method gives different results. Count the visible colonies on both plates separately. Choose the plates with 30–300 colonies.

Principle

Nitrogenous compounds, vitamin B complexes, and other growth nutrients are provided by yeast extract and the peptic digest of animal tissue. Agar acts as a solidifying agent.

Composition

Ingredients

Gms/Ltr

Peptic digest of animal tissue

5.00

Yeast extract

3.00

Agar

15.00

How to Prepare Yeast Extract Agar

TM Media, the leading manufacturer of Culture Media, offers Yeast Extract Agar (TM 496) with accurate composition of ingredients.



  • In 1000 mL of distilled water, dissolve 23 grams of dehydrated culture medium.
  • Bring the medium to a boil to completely dissolve it.
  • Autoclave at 15 psi pressure (121°C) for 15 minutes to sterilize the media.
  • Pour into sterile petri plates after thoroughly mixing.

Cultural Growth Observations

Microorganism

ATCC

Inoculum

(CFU/ml)

Growth

Enterobacter aerogenes

13048

50-100

Luxuriant

Escherichia coli

25922

50-100

Luxuriant

Pseudomonas aeruginosa

27853

50-100

Luxuriant

Staphylococcus aureus

25923

50-100

Luxuriant

At TM Media, we offer a wide range of culture media for bacterial or fungal isolation. Our product portfolio includes over 2000 different types of cultured media. Along with Culture Media, we also provide Laboratory Consumables, Biological Media Bases, Media Supplements, Antibiotic Sensitivity Discs, and many more.

For more, visit https://www.tmmedia.in/


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