Bacillus coagulans Isolation Using Glucose Yeast Extract BC Agar Medium

 

Bacillus coagulans is a lactic acid-producing bacterial species. It is a type of probiotic. Probiotics are live microorganisms that are intended to provide health benefits when consumed in the body. Probiotic organisms include Lactobacillus, Bifidobacterium, Enterococcus, Bacillus, and Streptococcus. Bacillus coagulans is a probiotic that produces lactic acid, but it is not the same as Lactobacillus. Unlike Lactobacillus and many other probiotics, it can produce spores during its reproductive life cycle. This ability allows Bacillus coagulans to go dormant during harsh conditions that would otherwise kill off other probiotics. As a result, this bacterial strain is particularly robust. It can withstand harsh environments, such as high levels of acid in the stomach. This may make Bacillus coagulans particularly effective at treating stomach upset and other ailments.

Spore forming Bacillus coagulans strains are used as probiotics for antibiotic-treated patients in some countries. The majority of probiotics lose their viability during harsh manufacturing processes and food storage. As a result, they must be encapsulated using special processes. Bacillus coagulans has the unusual ability to form spores, which aids in dormancy. Microbiological cultural procedures, as recommended by APHA, involve the use of primary recovery media and subculture media to identify spoilage bacteria and study their growth characteristics in routine diagnosis for spoilage in canned foods. Aerobic organism recovery media typically include Dextrose Tryptone Agar or Dextrose Tryptone Bromocresol Broth, whereas Cooked Meat Medium is recommended for anaerobic organism recovery.

Glucose Yeast Extract BC Agar Medium promotes sporulation, which is why it is used for the identification of mesophilic and thermophilic aerobic bacteria, such as Bacillus coagulans, in food and other materials.



Peptone and yeast extract in the medium provide nitrogenous and carbonaceous compounds, long chain amino acids, vitamins, and essential growth nutrients. To support Bacillus growth, a variety of salts, such as sulphates and phosphates, are used. Glucose (Dextrose) is a fermentable carbohydrate source. Manganese improves sporulation in Bacillus species.

Composition of Glucose Yeast Extract BC Agar Medium

Ingredients

Gms/Ltr

Peptone

5.0

Yeast extract

5.0

Dextrose (Glucose)

5.0

Di-Potassium hydrogen phosphate

0.5

Potassium dihydrogen phosphate

0.5

Magnesium sulphate

0.3

Trace mineral solution

1.0 ml

Agar

15.0

 

Trace mineral solution

mg/ml

Sodium chloride

10.0

Iron (II) sulfate, 7H2O

18.0

Manganese (II) sulfate, H2O

16.0

Zinc sulfate, 7H2O

1.6

Copper (II) sulfate, 5H2O

1.6

Cobalt (II) sulfate, 7H2O

1.6

TM Media provides a diverse range of Culture Media for bacterial or fungal isolation. In addition to Dehydrated Culture Media, they also sell Ready-to-Use Culture Media in plates, tubes, bottles, and bags. Their Culture Media collection contains over 2000 media, including R-2A Agar, Violet Red Bile Agar, Chromogenic UTI Agar, and many others. They also offer a veg range of various Culture Media as a sustainable alternative.

Along with Culture Media, they also provide Biological Media Bases, Media Supplements, Lab Chemicals, Plant Tissue Culture Media, Antibiotic Sensitivity Discs, Differentiation Discs, Culture Strains, Lab Consumables, Lab Equipment, Biological and Chemical Indicators, and many more microbiology products.

For more, visit https://www.tmmedia.in/.


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