Tryptone Soya Agar: An USP-Compliant Media

 

Tryptone Soya Agar, also known as Soyabean Casein Digest Agar, is a popular medium for the growth of a wide range of organisms, including pathogenic bacteria such as Neisseria, Listeria, and Brucella. The medium with blood addition provides perfectly defined haemolysis zones, while the sodium chloride content prevents erythrocyte lysis. It has been widely used in the pharmaceutical industry to create antigens, toxins, and other substances. Because of its simple and inhibitor-free composition, it is suitable for detecting antimicrobial agents in food and other products. Various pharmacopoeias recommend Tryptone Soya Agar as a sterility testing medium. Tryptone Soya Agar is USP-compliant and used in microbial limit testing and antimicrobial preservative testing. 



It is also used in the pharmaceutical industry to perform various tests such as environmental monitoring, maintenance of stock cultures, etc.

Principle

Gunn et al. used this medium to grow fastidious organisms and investigate the haemolytic reaction after the addition of 5% v/v blood. This medium is nutritious due to the combination of tryptone and soy peptone, which provide amino acids and long-chain peptides for the growth of microorganisms. The osmotic balance is maintained by sodium chloride. X and V growth factors are not present in Tryptone Soya Agar. It is simple to use in determining the requirements of these growth factors by Haemophilus isolates by adding X-factor, V-factor, and X+V factor discs to inoculated Tryptone Soya Agar plates.

Composition

Ingredients

Gms / Ltr

Pancreatic digest of casein

15.000

Soya peptone

5.000

Sodium chloride

5.000

Agar

15.000

How To Prepare TSA

  • In 1000 mL of purified or distilled water, dissolve 40 grams of TM Media TSA.
  • To completely dissolve it, bring the medium to a boil.
  • Sterilize by autoclaving at 15 psi (121°C) for 15 minutes.
  • If desired, aseptically add 5% v/v sterile defibrinated blood to a previously cooled medium to 45–50 °C for cultivation.
  • Pour it into sterile petri plates after thoroughly mixing.
  • Light yellow-colored, clear to slightly opalescent gel media is observed in plates.
  • If added 5-7% w/v sterile defibrinated blood to petri plates, a cherry-red, opaque gel forms.

Instruction For Use

  • After collection, inoculate the specimen as soon as possible.
  • Inoculate the sample on the petri plate by streaking or swabbing.
  • Incubate plates aerobically at 35–37 °C for 18–24 hours.
  • Examine the colonies identification.

For environmental monitoring in the clean room environment, we offer our range Absolute EM which are ready-to-use culture media plates which are triple packed and gamma-irradiated.

At TM Media, we also offer a wide range of culture media for bacterial or fungal isolation. Over 2,000 Dehydrated Culture Media are available in our portfolio. Culture Media is also available as a Ready-To-Use product. The Read-To-Use Culture Media collection is available in plates, tubes, bottles, and bags. Along with Culture Media, we also provide Laboratory Consumables, Biological Media Bases, Media Supplements, Antibiotic Sensitivity Discs, and many more.

For more, visit https://www.tmmedia.in/.

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