Plate Count Agar: A General Media For Sanitary Testing


Plate Count Agar, also known as Standard Methods Agar, is a microbiological growth medium that is commonly used to assess or monitor a sample's "total" or viable bacterial growth. Plate Count Agar is not a selective medium. It can be used to determine microorganism plate counts from a variety of samples, including foods, water, wastewater, and clinical samples. It is also used to count the number of microorganisms in other sanitary materials. This medium was formulated and described by Buchbinder et al. Plate Count Agar meets the requirements of ISO 4833-1 and 2:2013, as well as APHA. In this non-selective medium, the bacteria are counted as colony-forming units per gram (CFU/g) in solid samples and CFU/ml in liquid sam

                                                   Composition of Plate Count Agar

Ingredients

Gms/Ltr

Agar

15.00

Tryptone

5.00

Yeast extract

2.50

Dextrose

1.00

Principle



The medium consists of tryptone, which provides nitrogen, vitamins, minerals, and amino acids essential for the growth of microorganisms. Yeast extract is a source of vitamins, particularly the B group. Dextrose is a fermentable sugar, that provides carbon and energy. Agar acts as a solidifying agent.

Instruction For Use

  • In 1000 ml of distilled water, dissolve 23.50 grams.
  • Bring to a gentle boil while gently swirling to dissolve the medium.
  • Autoclave at 15 psi (121 °C) for 15 minutes to sterilise.
  • Allow cooling to 45-50 degrees Celsius.
  • Mix thoroughly and pour into sterile Petri dishes.

Benefits Of Using Plate Count Agar:

  • Easy to prepare
  • The number of microbes per milliliter in a specimen can be determined.
  • Bacterial contamination can be detected in a wide range of samples.

Colony Characteristics

Microorganism

ATCC

Inoculum

(CFU/ml)

Growth

Bacillus subtilis

6633

50-100

Luxuriant

Escherichia coli

25922

50-100

Luxuriant

Lactobacillus casei

9595

50-100

Luxuriant

Staphylococcus aureus

25923

50-100

Luxuriant

Staphylococcus pyogenes

19615

50-100

Luxuriant

Enterococcus faecalis

29212

50-100

Luxuriant

At TM Media, we also offer a wide range of culture media for bacterial or fungal isolation. Over 2,000 Dehydrated Culture Media are available in our portfolio. Culture Media is also available as a Ready-To-Use product. The Read-To-Use Culture Media collection is available in plates, tubes, bottles, and bags. Along with Culture Media, we also provide Laboratory Consumables, Biological Media Bases, Media Supplements, Antibiotic Sensitivity Discs, and many more.

For more, visit https://www.tmmedia.in/.


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