Peptone: An Important Component Of Culture Media

 


Nitrogen is an essential nutrient for the growth of microorganisms. It is primarily required for the synthesis of amino acids, DNA, RNA, and ATP, among others. Nitrogen enables bacteria to synthesise proteins.

Proteins have complex structures and a wide range of types, so their hydrolysates have similar properties. In biological culture, protein-containing raw materials are referred to as "organic nitrogen sources."

The organic form of nitrogen is proteose peptone or tryptone. Nitrates are the inorganic form of nitrogen.

Peptone

Peptone contains polypeptides and amino acids and serves as a readily available source of nitrogen and minerals for bacterial growth. Peptone contains a large amount of tryptophan. It is used in a variety of media as a cultural media ingredient. It can also be used to make enzymes, vaccines, antibiotics, steroids, and other products for commercial use.

Peptone is synthesised through the controlled enzymatic hydrolysis of animal tissues. It is used in microbiological culture media as an organic nitrogen source for the cultivation of various bacteria and fungi. It is a protein derivative produced by the partial hydrolysis of proteins in an acidic environment. It is not coagulated by heat and is water-soluble.



Peptones are commonly used in culture media optimization for bacterial growth microbial cell culture processes. Peptones are continuing to be successfully used to improve the production of monoclonal antibodies, biosimilars, and vaccines. It is also used as a nutrient in industrial fermentation (for example, vaccine production) to overcome the lag phase.

Peptones are ideal for media containing blood for haemolytic studies (pneumococci, streptococci). It is an ingredient in Moeller's Decarboxylase Test Medium, Moeller's KSGN Broth, Voges-Proskauer Substrate, and Sabouraud Substrate.

Sources of peptone

Peptone is produced through the incomplete hydrolysis of proteins derived from beef, casein, milk powder, gelatin, soy protein, silk protein, fibrin, and other sources. The majority of commercially available products are powders that range from light yellow to brownish yellow. It has a molecular weight somewhere between proteose and peptide.

TM Media, Manufacturer Of Peptone

TM Media offers a wide range of Peptones. Our portfolio includes both animal and plant-based peptones such as Veg Bio-peptone, Bio-peptone, Fish Peptone, Peptone-R, Wheat Peptone, and many more peptone variants.

Several commercially available peptones are used as growth supplements in various cell culture processes. Different types of peptone supplements are used depending on the type of cell culture, such as plant, animal, or microbial.

Though the composition of hydrolysate varies greatly depending on the protein source and hydrolysis conditions, peptone is frequently a complex mixture of peptides. Peptone contains many other components besides amino acids, so it can provide carbon sources, nitrogen sources, growth factors, and other nutrients for biological cultivation and fermentation.

Tryptone, meat peptone, bone peptone, silk pupae peptone, blood peptone, fish and shrimp powder, and milk powder are some animal proteins that are commonly used.

Vegetable peptones are made from peanut and soybean meals. The sugar content of vegetable peptone is relatively high.

For more details about our products, visit https://www.tmmedia.in/.

Comments

Post a Comment

Popular posts from this blog

Swabs With Transport Medium: An Overview With Product Examples

  To treat a microbial infection, an accurate diagnosis is crucial. In a clinical laboratory, the accuracy of a medical diagnosis begins with two key components: the right tool for collecting a sufficient amount of a sample and careful storage of that sample until it is transferred to a laboratory for analysis. Microbial samples can easily degrade if not properly stored during transport. This is why it is critical to choose the appropriate transport culture media. In this article, we will define transport media and its components, as well as their varieties and uses. What is a transport medium? Transport media are a solution for safely transporting microbial samples to a laboratory for analysis. It is a liquid, balanced buffer solution containing carbohydrates, peptones, and other essential nutrients, except growth factors. A transport medium is used to keep bacteria viable during transport while preventing them from multiplying. Without an appropriate transport medium and temperature,
Read more

Antibiotic Sensitivity Discs – The Easiest Way To Find The Treatment For Microbial Infections

Image
  You've probably heard of strep throat, the common cold, and ringworms. These are some common microbial infections. Micro-organisms, as their name suggests, are organisms or living beings that are so small that they cannot be seen by naked eyes. Micro-organisms are ubiquitous, which means they are present everywhere in air, water, food, even on human skin or inside their guts. Not all microorganisms are harmful; some are beneficial too. Microorganisms play a significant role in various industries and are even used to formulate antibiotics. I know it may sound strange to you that microbes produce antibiotics because we use antibiotics to kill microbes. Yes, antibiotics are compounds produced by microbial species to kill or inhibit the growth of other bacterial species when given at a specified concentration. That’s why when you go to a doctor, he gives you an antibiotic or a set of antibiotics. After administration, antibiotics can kill or inhibit microbes by destroying their cell
Read more

Tryptone Soya Agar: An USP-Compliant Media

Image
  Tryptone Soya Agar, also known as Soyabean Casein Digest Agar, is a popular medium for the growth of a wide range of organisms, including pathogenic bacteria such as Neisseria, Listeria, and Brucella. The medium with blood addition provides perfectly defined haemolysis zones, while the sodium chloride content prevents erythrocyte lysis. It has been widely used in the pharmaceutical industry to create antigens, toxins, and other substances. Because of its simple and inhibitor-free composition, it is suitable for detecting antimicrobial agents in food and other products. Various pharmacopoeias recommend Tryptone Soya Agar as a sterility testing medium. Tryptone Soya Agar is USP-compliant and used in microbial limit testing and antimicrobial preservative testing.  It is also used in the pharmaceutical industry to perform various tests such as environmental monitoring, maintenance of stock cultures, etc. Principle Gunn et al. used this medium to grow fastidious organisms and investig
Read more