Quick And Easy Detection Of UTI Causative Agents

Urinary tract infections, as the name suggests, are infections that affect the urinary tract. Urgency and frequency of urination, along with any resulting discomfort or pain, are typical symptoms of a urinary tract infection. The most common condition is cystitis, which is brought on by an infection of the bladder with a uropathogenic bacterium, most frequently Escherichia coli, but also occasionally Staphylococcus saprophyticus, Klebsiella species, Proteus mirabilis, other coliforms, Pseudomonas aeruginosa, or Enterococcus faecalis, especially in hospital-acquired infections.

Early detection is the key to any treatment. Chromogenic UTI Agar is specifically formulated to identify UTI-causing organisms easily. Based on research by Pezzlo Wilkie et al., Friedman et al., Murray et al., Soriano and Ponte, and Merlino et al., Chromogenic UTI Agar was developed. It is known for its rapidity, accuracy, and simplicity during diagnosis. It saves time and labour. When compared to traditional methods, chromogenic media yields faster results for both negative screens and presumptive positives. Chromogenic culture media are cost-effective because, on a single medium, one can identify more than one organism on the basis of colony color. As a result, the results can be interpreted without having to go through the traditional identification process, such as biochemical tests.



Chromogenic UTI Agar is a differential medium used to identify the microorganisms responsible for urinary tract infections. It contains chromogens, the chromogenic substrate. The chromogenic substrates and specific enzymes produce different contrasted colony colors due to bacterial reactions, and the media makes it easier to identify bacterial colonies. For instance, two specific chromogenic substrates are present in the medium and are broken down by enzymes produced by Enterococcus species, Escherichia coli, and coliforms.

TM Media, the strategic business unit of Titan Biotech Ltd., offers a wide range of Chromogenic Culture Media in both Dehydrated and Ready-to-Use forms. They have over 100 Chromogenic Culture Media. They have Chromogenic Culture Media for a variety of microbes, such as Chromogenic Coliform Agar Plate (TMP 1858), Chromogenic E. coli Agar Plate Triple Pack (TMP 073T), Chromogenic UTI Agar Plate (TMP 1199), Chromogenic ECC Agar (TM 1340), Chromogenic E. coli Agar (TM 2119), Chromogenic Enterobacter sakazakii Agar (TM 1631), Chromogenic Klebsiella Selective Agar Base (TM 1633), Chromogenic Listeria Agar Base (TM 004), and many more.

Composition of TM Media’s Chromogenic UTI Agar

Ingredients

Gms / Ltr

Peptone (special)

15.00

Chromogenic mixture

2.45

Agar

15.00

Peptone special provides nitrogenous and carbonaceous compounds and other essential growth nutrients, while agar acts as a solidifying agent. Chromogenic UTI Agar contains two specific Chromogenic substrates that are cleaved by enzymes produced by Enterococcus spp., Escherichia coli, and coliforms. In addition, it contains phenylalanine and tryptophan, which provide an indication of tryptophan deaminase activity, indicating the presence of Proteus spp. and Morganella spp. and Providencia spp.

How To Use TM Media’s Chromogenic UTI Agar

  • Dissolve 32.45 grams of TM Media’s Chromogenic UTI Agar in 1000 ml of distilled water.

  • Gently heat to boiling while swirling to dissolve the medium completely.

  • Sterilize by autoclaving at 15 psi (121°C) for 15 minutes.

  • Cool to 45–50 °C.

  • Pour into sterile Petri dishes after thoroughly mixing.

Growth Characteristics

The Enterococci form blue colonies when the enzyme ß-glucosidase cleaves one of the chromogenic substrates. The ß-D-galactosidase enzyme, which cleaves the other Chromogenic substrate, is what causes Escherichia coli to produce pink colonies. Coliforms create purple colonies by cleaving both the Chromogenic substrate. Tryptophan deaminase activity causes the colonies of Proteus spp., Morganella spp., and Providencia spp. to appear brown.

At TM Media, they offer a wide range of culture media for bacterial or fungal isolation. The Culture Media collection is available in two formats: Dehydrated and Ready-to-Use. The Ready-to-Use Culture Media is available in different packaging, including Petri plates of 90 mm and 55 mm sizes, tubes, bottles, and 5-liter bags. Along with Culture Media, they also provide Biological Media Bases, Media Supplements, Lab Chemicals, Plant Tissue Culture Media, Antibiotic Sensitivity Discs, Differentiation Discs, Culture Strains, Lab Consumables, Lab Equipment, Biological and Chemical Indicators, and many more microbiology products.

For more, visit https://www.tmmedia.in/.

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