Grow Yeast with The Accurate Composition of MGYP Medium

Introduction Yeast extract agar is a highly nutritive medium containing yeast extract as a source of vitamins. It allows the cultivation of a wide spectrum of bacteria, yeasts, and molds. Yeast extract agar is formulated according to the formula described by Windle Taylor for the plate count of microorganisms in water. A large number of microorganisms, particularly those from soil and vegetation, can be found in water. This medium can also be used for teaching and demonstrations with simple organisms. Make a decimal dilution bank with Ringer Solution from the water sample and transfer aliquots to two parallel series of plates. Pour in the cooled molten Yeast Extract Agar and homogenise with the sample. Incubate one of the plates for 24 hours at 35°C and the other plate for 3 days at 20–22°C. This method gives different results. Count the visible colonies on both plates separately. Choose the plates with 30–300 colonies. Principle Nitrogenous compounds, vitamin B complexes, an

  Thiosulfate-citrate-bile salts-sucrose (TCBS) Agar is used in standard food testing methods and clinical specimen testing to isolate and detect Vibrio cholerae and Vibrio parahaemolyticus . Kobayashi developed TCBS agar by modifying Nakanishi's selective medium. This highly selective medium meets the nutritional needs of Vibrio species while also allowing them to compete with intestinal flora. This medium isolates the Vibrio species based on their ability to grow at an alkaline pH and high salt concentration. In cases of gastroenteritis caused by Campylobacter species, whose symptoms mimic those of cholera, the TCBS becomes extremely important. As no yellow bacterial growth is observed in the case of Campylobacter species on TCBS agar, the possibility of an incorrect diagnosis can be eliminated Composition Ingredients Gm/L Yeast extract 5.0 Proteose Peptone 10.0 Sodium thiosulfate 10.0

  Agar, also known as agar-agar, is a jelly-like substance made up of polysaccharides extracted from the cell walls of various red algae, primarily ogonori (Gracilaria) and "tengusa" (Gelidiaceae). Agar is composed of two components: the linear polysaccharide agarose and a heterogeneous mixture of smaller molecules known as agaropectin. The agarose makes up about 70% of the mixture. Agarose is a linear polymer composed of repeating agarobiose units, a disaccharide composed of D-galactose and 3,6-anhydro-L-galactopyranose. Agaropectin is a heterogeneous mixture of smaller molecules found in lower concentrations. It is composed of alternating units of D-galactose and L-galactose that have been heavily modified with acidic side groups such as sulphate and pyruvate. The mixture forms a supporting structure in the cell walls of certain algae species and is released when heated. Agarophytes are algae that belong to the Rhodophyta (red algae) phylum. The agaropectin is removed duri

  Bacillus coagulans is a lactic acid-producing bacterial species. It is a type of probiotic. Probiotics are live microorganisms that are intended to provide health benefits when consumed in the body. Probiotic organisms include Lactobacillus, Bifidobacterium, Enterococcus, Bacillus, and Streptococcus. Bacillus coagulans is a probiotic that produces lactic acid, but it is not the same as Lactobacillus. Unlike Lactobacillus and many other probiotics, it can produce spores during its reproductive life cycle. This ability allows Bacillus coagulans to go dormant during harsh conditions that would otherwise kill off other probiotics. As a result, this bacterial strain is particularly robust. It can withstand harsh environments, such as high levels of acid in the stomach. This may make Bacillus coagulans particularly effective at treating stomach upset and other ailments. Spore forming Bacillus coagulans strains are used as probiotics for antibiotic-treated patients in some countries.