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Showing posts from March, 2023

Find The Best Treatment for Microbial Infections with Antibiotic Sensitivity Discs

  You must have taken antibiotics once in your life. Do you ever think about how antibiotics work? How can you predict whether an antibiotic will be effective or not in treating a bacterial infection? Yes, but can’t find the answer. Don’t worry! Because in this article we will discuss this question. Before answering these, let's first define antibiotics. An antibiotic is a compound produced by a microbial species to kill or inhibit the growth of other bacterial species when given in a specified concentration. Now, the Action Mechanism of Antibiotics After administration, antibiotics can kill or inhibit bacteria by destroying their cell walls, inhibiting cell wall synthesis, producing toxins, disturbing the metabolic pathway, and inhibiting replication, transcription, and translation of microbes. So now the question is, how can you predict whether an antibiotic will be effective or not in treating a bacterial infection? The answer is: antibiotic sensitivity test. Antibiotic Sensitiv

Swabs With Transport Medium: An Overview With Product Examples

  To treat a microbial infection, an accurate diagnosis is crucial. In a clinical laboratory, the accuracy of a medical diagnosis begins with two key components: the right tool for collecting a sufficient amount of a sample and careful storage of that sample until it is transferred to a laboratory for analysis. Microbial samples can easily degrade if not properly stored during transport. This is why it is critical to choose the appropriate transport culture media. In this article, we will define transport media and its components, as well as their varieties and uses. What is a transport medium? Transport media are a solution for safely transporting microbial samples to a laboratory for analysis. It is a liquid, balanced buffer solution containing carbohydrates, peptones, and other essential nutrients, except growth factors. A transport medium is used to keep bacteria viable during transport while preventing them from multiplying. Without an appropriate transport medium and temperature,

A Chocolate - Not For Humans

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  Introduction Chocolate agar, also known as chocolate blood agar, is a nonselective, enriched growth medium used for the isolation of fastidious pathogenic bacteria. It is a variant of blood agar that contains lysed red blood cells. The red blood cells have been lysed by slow heating to 80°C; on heating, the colour of the medium becomes chocolate-brown. Hence, the name of the agar is derived from its colour, and it does not contain any chocolate products. The lysed red blood cells release nutrients that help fastidious bacteria grow, most notably Haemophilus and Neisseria species. With the addition of bacitracin, chocolate agar becomes selective for the genus Haemophilus. Thayer-Martin agar is a chocolate agar variant that contains an assortment of antibiotics that select for Neisseria species. Principle Microorganisms such as Haemophilus influenzae require growth factors such as nicotinamide adenine dinucleotide (factor V or NAD) and hemin (factor X), which are found inside red bloo

How To Use Blood Agar Plates

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  Are you looking for a reliable, easy-to-use method for growing bacteria? If so, you need a blood agar plate. Blood agar is an enriched type of growth media that contains 5% blood supplement. This makes it an ideal environment for growing fastidious bacteria. In this article, we will explain how to use blood agar plates to grow bacteria. We will also provide tips on how to store ready-to-use blood agar plates. Blood Agar Sheep Blood Agar is a differential as well as an enriched medium. It is used to isolate, cultivate, and detect the haemolytic activity of pathogenic micro-organisms such as Streptococci and Pneumococci. The source of animal blood and the type of base medium used can influence haemolytic patterns. Nucleotidase destroys V factors in sheep blood, preventing Haemophilus species from growing on sheep blood agar unless other micro-organisms, such as Staphylococci, provide the V factors. Small amounts of reducing sugars inhibit the expression of β-haemolysis. Streptococci t

Antibiotic Sensitivity Discs – The Easiest Way To Find The Treatment For Microbial Infections

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  You've probably heard of strep throat, the common cold, and ringworms. These are some common microbial infections. Micro-organisms, as their name suggests, are organisms or living beings that are so small that they cannot be seen by naked eyes. Micro-organisms are ubiquitous, which means they are present everywhere in air, water, food, even on human skin or inside their guts. Not all microorganisms are harmful; some are beneficial too. Microorganisms play a significant role in various industries and are even used to formulate antibiotics. I know it may sound strange to you that microbes produce antibiotics because we use antibiotics to kill microbes. Yes, antibiotics are compounds produced by microbial species to kill or inhibit the growth of other bacterial species when given at a specified concentration. That’s why when you go to a doctor, he gives you an antibiotic or a set of antibiotics. After administration, antibiotics can kill or inhibit microbes by destroying their cell

Indole-3-Butyric Acid (IBA) - A Product For Successful Research

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  Hormones play an important role in the human body. They control and coordinate all of the body's functions, from metabolism to growth and development, and even human emotions, mood, and sleep. Any changes in hormone deficiency or excess result in a physiological change. Similarly, like in humans, hormones control all aspects of plant growth and development. Environmental cues trigger the production of these potent hormone compounds. They are found in the leaves, flowers, shoots, roots, or fruits, depending on which hormone is activated. Plant hormones are classified into five types: auxins, cytokinins, gibberellins, ethylene, and abscisic acid. Each hormone serves a specific purpose, and for oilseed, pulse, and cereal crops, auxins and cytokinins can significantly improve plant vigour, promote root and shoot growth, and reduce stress. In this article, we will discuss auxin, identified as indole-3-butyric acid (IBA). Auxins You all have most likely seen auxins in action, but few o